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Granzyme K, PerCP-eFluor™ 710, clone: G3H69, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-8897-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15509386

  • £77.07 / 25 tests

EU Stock 2
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Description: This G3H69 monoclonal antibody reacts with human Granzyme K. Granzyme K is one of five granzyme serine proteases that have been identified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Granzyme A and Granzyme K are both tryptases and appear to show overlapping, though not identical, substrate specificity. This functional similarity is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs.Applications Reported: This G3H69 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This G3H69 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


Granzyme K
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
IgG2a, kappa
Flow Cytometry
PerCP-eFluor 710
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.