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Granzyme K Mouse anti-Human, eFluor™ 660, Clone: G3H69, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-8897-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540057

  • £74.50 / 25 tests

Estimated Shipment: 25-10-2021

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Description

Description

Description: This G3H69 monoclonal antibody reacts with human Granzyme K. Granzyme K is one of five granzyme serine proteases that have been indentified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Granzyme A and Granzyme K are both tryptases and appear to show overlapping, though not identical, substrate specificity. This functional similarity is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs. Applications Reported: This G3H69 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This G3H69 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
Specifications

Specifications

Granzyme K
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P49863
GrK
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
G3H69
eFluor 660
GZMK
Liquid
GZMK
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3003
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For Research Use Only.