Granzyme B Rat anti-Mouse, Clone: 16G6, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8822-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15258097

Quantity Price
1 £ 158.36 / 100µg
EU Stock 1
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Description and Specification

Specification

Primary or Secondary Primary
Conjugate Unlabeled
Quantity 100μg
Species Reactivity Mouse
Format Purified
Applications ELISA Capture
Applications Immunohistochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Western Blotting
Storage Requirements Store at 2-8°C.
Antigen Granzyme B
Isotype IgG2b
Host Species Rat
Monoclonal or Polyclonal Monoclonal
Concentration 0.5mg/mL
Clone 16G6
Regulatory Status RUO
Gene Alias GrzB, GrB
Formulation aqueous buffer, 0.09% sodium azide, contains carrier protein/stabilizer if necessary

The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.

For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com.