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Granzyme A, PE-Cyanine7, clone: GzA-3G8.5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-5831-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15548576

  • £199.23 / 100µg


Estimated Shipment date
from Supplier 26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This Gza-3G8.5 monoclonal antibody reacts with mouse Granzyme A. Granzyme A is the most abundantly expressed of the ten granzyme serine proteases that have been identified in mice. Granzymes are proteins released from the granules of NK cells and cytotoxic T lymphocytes that induce death in target cells by cleavage of intracellular substrates and play a critical role in immune defense against viruses, tumors, and intracellular bacteria. Granzyme A activates caspase-independent cell death pathways morphologically similar to apoptosis and characterized by mitochondrial and DNA damage. It may also play a role in inflammation, as the precursor form of IL-1 beta (pro-IL-1 beta) is among its target substrates. Granzyme A shares overlapping substrate specificity with the closely-related Granzyme K, which is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs.Applications Reported: This GzA-3G8.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This GzA-3G8.5 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Granzyme A
GzA-3G8.5
PE-Cyanine7
Conjugated
Murine
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CTLA-3, Fragmentin-1, TSP-1
IgG2b
RUO
Primary
Murine
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