missing translation for 'onlineSavingsMsg'
Learn More

Granzyme A, PE, clone: CB9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9177-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15527186

  • £75.29 / 25 tests


EU Stock 1
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: This CB9 monoclonal antibody reacts with human Granzyme A. Granzyme A is the most abundantly expressed of the granzyme serine proteases, which are proteins released from the granules of NK cells and cytotoxic T lymphocytes that induce death in target cells by cleavage of intracellular substrates. They play a critical role in immune defense against viruses, tumors, and intracellular bacteria. Granzyme A activates caspase-independent cell death pathways morphologically similar to apoptosis and characterized by mitochondrial and DNA damage. It may also play a role in inflammation, as the precursor form of IL-1 beta (pro-IL-1 beta) is among its target substrates. Granzyme A shares overlapping substrate specificity with the closely-related Granzyme K, which is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTL.Applications Reported: This CB9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This CB9 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood lymphocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Granzyme A
CB9
PE
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CTLA-3, Fragmentin-1, TSP-1
IgG1
RUO
Primary
Human
Videos