Invitrogen™ Granzyme A Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS2232

Product Code. 15964204

Description

The Human Granzyme A ELISA quantitates Hu Granzyme A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme A.

Principle of the method

The Human Granzyme A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Granzyme A (GzmA) is the most abundant serine protease in killer cell cytotoxic granules. GzmA activates a novel programmed cell death pathway that begins in the mitochondrion, where cleavage of NDUFS3 in electron transport complex I disrupts mitochondrial metabolism and generates reactive oxygen species (ROS). GzmA also targets other important nuclear proteins for degradation, including histones, the lamins that maintain the nuclear envelope, and several key DNA damage repair proteins (Ku70, PARP-1). GzmA also has proinflammatory activity; it activates pro-interleukin-1? and may also have other proinflammatory effects that remain to be elucidated.

Specifications

• Accession Number: P12544
• Assay Sensitivity: 0.73 pg/ml
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 3001
• Interassay CV: 8.5%
• Kit Contents: Pre-coated 96-well Plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromagen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Serum, 50 μL; Plasma, 50 μL; Cell Lysate,50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 7.8-500 pg/ml
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GZMA
• Intraassay CV: 4.9%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.