GPNMB Mouse anti-Human, PE, Clone: HOST5DS, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-9838-41

25TEST Anti-Human GPNMB PE

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15567236

Quantity Price
1 £ 101.6 / 25 tests
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

Antigen GPNMB
Applications Flow Cytometry
Clone HOST5DS
Concentration 5μL (0.125μg)/test
Conjugate PE
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Osteoactivin, DC-HIL, HGFIN
Host Species Mouse
Isotype IgG1
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This HOST5DS monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells, and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. THP-1 and U937 cell lines constitutively express GPNMB. GPNMB may also be secreted.

The HOST5DS antibody will work on paraformaldehyde-fixed and permeabilized cells, and can be used for intracellular staining. Please, note that enzymatic tissue dissociation may reduce the amount of surface GPNMB, and thereby negatively impact the brightness of the staining.