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GPNMB, PE, clone: HOST5DS, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9838-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15577236

  • £162.37 / 100 tests


EU Stock 2
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Disclaimers:

For Research Use Only.


Description

Description

Description: This HOST5DS monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells, and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. THP-1 and U937 cell lines constitutively express GPNMB. GPNMB may also be secreted.The HOST5DS antibody will work on paraformaldehyde-fixed and permeabilized cells, and can be used for intracellular staining. Please, note that enzymatic tissue dissociation may reduce the amount of surface GPNMB, and thereby negatively impact the brightness of the staining.Applications Reported: This HOST5DS antibody has been reported for use in flow cytometric analysis.Applications Tested: This HOST5DS antibody has been pre-titrated and tested by flow cytometric analysis of human blood monocyte-derived macrophages. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Tissue dissociation protocols involving enzymatic digestion should be optimized to limit proteolytic degradation of GPNMB on the cell surface.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GPNMB
HOST5DS
PE
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Osteoactivin, DC-HIL, HGFIN
IgG1
RUO
Primary
Human
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