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GM-CSF, PE, clone: MP1-22E9, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7331-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15299319

  • £137.15 / 100µg


EU Stock 4
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Description

Description

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MP1-22E9 antibody is tested by intracellular staining and flow cytometric analysis of cultured mouse splencoytes. It is offered in 2 formats: -μg size: has been tested bu intracellular staining and flow cytometric analysis of cultured mouse splencoytes. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested bu intracellular staining and flow cytometric analysis of cultured mouse splencoytes. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GM-CSF
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01587
Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MP1-22E9
PE
Csf2
Conjugated
Csf2
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12981
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