GM-CSF, FITC, clone: MP1-22E9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-7331-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF alpha, IL-2, IL-1, and IFN gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MP1-22E9 antibody is offered in 2 formats: - μg size: has been tested. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|0.5mg/mL; 5μL (0.25μg)/test|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Granulocyte-macrophage colony stimulating factor, CSF2|
For Research Use Only.