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GM-CSF, FITC, clone: MP1-22E9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-7331-81
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF alpha, IL-2, IL-1, and IFN gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MP1-22E9 antibody is offered in 2 formats: - μg size: has been tested. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
GM-CSF | |
Monoclonal | |
0.5mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P01587 | |
Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin | |
Rat | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
MP1-22E9 | |
FITC | |
Csf2 | |
Conjugated | |
Csf2 | |
IgG2a, kappa | |
50μg | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
12981 |
For Research Use Only.