GM-CSF, FITC, clone: MP1-22E9, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-7331-81

Product Code. 15238369

Description

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF alpha, IL-2, IL-1, and IFN gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MP1-22E9 antibody is offered in 2 formats: - μg size: has been tested. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: GM-CSF
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01587
• Gene Alias: Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: MP1-22E9
• Conjugate: FITC
• Gene: Csf2
• Format: Conjugated
• Gene Symbols: Csf2
• Isotype: IgG2a, kappa
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 12981

For Research Use Only.