GM-CSF, Functional Grade, clone: MP1-22E9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7331-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: The MP1-22E9 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.Applications Tested: The Functional Grade Purified MP1-22E9 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity. The MP1-22E9 antibody at 1.25 μg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse GM-CSF in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The MP1-22E9 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the biotin MP1-31G6 (13-7332) antibody for detection and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin|
|Cytokine BioassaysþCytokine NeutralizationþELISA|
|Store at 2-8°C.|
For Research Use Only.