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GM-CSF, clone: MP1-22E9, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7331-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218077

  • £151.42 / 500µg


Estimated Shipment
29-06-2018
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Description

Description

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: This MP1-22E9 antibody has been reported for use in capture of mouse GM-CSF by ELISA, intracellular staining and flow cytometric analysis, neutralization of GM-CSF bioactivity, western blotting, and immunohistochemical staining of frozen tissue sections.Applications Tested: The MP1-22E9 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the biotin MP1-31G6 (13-7332) antibody for detection and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GM-CSF
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P01587
Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Western Blot
MP1-22E9
Unconjugated
Csf2
Purified
Csf2
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
12981
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