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GM-CSF, PE, clone: DAVKAT, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7209-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15567106

  • £75.39 / 25 tests


EU Stock 1
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Description

Description

Description: This DAVKAT monoclonal antibody reacts with human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). Human GM-CSF is a 127 amino acid, differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with the extracellular matrix and in membrane-bound formats as well. It stimulates proliferation, activation and differentiation of macrophages, granulocytes and their progenitors.Applications Reported: This DAVKAT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This DAVKAT antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GM-CSF
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P04141
Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
DAVKAT
PE
CSF2
Conjugated
CSF2
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
1437
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