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GP6, eFluor 660, clone: HY101, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9813-41

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15500147

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Description

Description

Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets. Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke.

Description: The HY101 monoclonal antibody reacts with the glycoprotein VI (GPVI), a 62-kDa type-I transmembrane glycoprotein that is expressed on mature megakaryotes and platelets. GPVI associates with the Fc receptor (FcR) gamma-chain to form a high affinity receptor for collagen. GPVI has also been reported to bind convulxin (Cvx). Phosphorylation of the ITAMs present on the FcR gamma-chain leads to recruitment of Src family kinases and activation of downstream signal transduction. GPVI has been reported to play a critical role in platelet activation and aggregation.Applications Reported: This HY101 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HY101 antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GP6
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9HCN6
GP6
Affinity chromatography
RUO
51206
Store at 2-8°C. Do not freeze. Light-sensitive material.
Liquid
Flow Cytometry
HY101
eFluor 660
GP6
BDPLT11, GPIV, GPVI, glycoprotein 6, platelet collagen receptor, platelet glycoprotein VI
Mouse
25 Tests
Primary
Human
Antibody
IgG1 κ
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For Research Use Only.