GFAP, eFluor 615, clone: GA5, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 42-9892-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.Applications Reported: This GA5 antibody has been reported for use in immunocytochemical (ICC) and immunohistochemical staining of frozen tissue sections (IHC-F).Applications Tested: This GA5 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 10 μg/mL. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|Glial Fibrillary Acid Protein (GFAP)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|P14136, P03995, P47819|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.