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GFAP, eFluor 615, clone: GA5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-9892-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15301130

  • £70.36 / 25µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.Applications Reported: This GA5 antibody has been reported for use in immunocytochemical (ICC) and immunohistochemical staining of frozen tissue sections (IHC-F).Applications Tested: This GA5 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 10 μg/mL. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Glial Fibrillary Acid Protein (GFAP)
GA5
eFluor 615
Conjugated
IgG1
RUO
Primary
Rat
Microscopy
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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