GFAP Monoclonal Antibody (GA5), eFluor™ 615, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15301130	Affymetrix eBioscience 42-9892-80	25 μg
15311130	Affymetrix eBioscience 42-9892-82	100 μg

Description

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predomit form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in immunocytochemical (ICC) and immunohistochemical staining of frozen tissue sections (IHC-F). Applications Tested: This GA5 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 10 μg/mL. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75).

GFAP Monoclonal antibody specifically detects GFAP in Chicken, Human, Mouse, Porcine, Rabbit, Rat samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen).

Specifications

• Antigen: GFAP
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
• Gene Symbols: GFAP
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: GFAP

• Clone: GA5
• Applications: Immunocytochemistry, Immunohistochemistry (Frozen)
• Conjugate: eFluor 615
• Host Species: Mouse
• Target Species: Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Gene Accession No.: P03995, P14136, P47819
• Gene ID (Entrez): 100352532, 14580, 24387, 2670, 396562, 419969
• Isotype: IgG1
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.