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GFAP Monoclonal Antibody (GA5), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-9892-82
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predomit form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry. Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
GFAP Monoclonal antibody specifically detects GFAP in Chicken, Human, Mouse, Porcine, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).Specifications
GFAP | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P03995, P14136, P47819 | |
GFAP | |
Affinity chromatography | |
RUO | |
100352532, 14580, 24387, 2670, 396562, 419969 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) | |
GA5 | |
Alexa Fluor 488 | |
GFAP | |
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen | |
Mouse | |
100 μg | |
Primary | |
Chicken, Human, Mouse, Porcine, Rabbit, Rat | |
Antibody | |
IgG1 |
For Research Use Only.