GL7 Monoclonal Antibody (GL-7 (GL7)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5902-82

Product Code. 15539316

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.

GL7 Monoclonal antibody specifically detects GL7 in Human, Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: GL7
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: GL-7 (GL7)
• Conjugate: PerCP-eFluor 710
• Gene Alias: GL7 antigen; Ly77; Ly-77
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgM

For Research Use Only.