GILZ, PE, clone: CFMKG15, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-4033-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB. GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predominantly cytoplasmic staining.Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.Applications Tested: This CFMKG15 antibody has been tested by intracellular flow cytometric analysis of dexamethasone-treated mouse lymph node cells and human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For intracellular staining with CFMKG15, it is recommended to use IC Fixation Buffer (cat. 00-8222) followed by permeabilization with a 1X dilution of the 10X Permeabilization Buffer (cat. 00-8333).Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TSC22D3, TSC22 domain family member 3, TSC-22 related protein, delta sleep inducing peptide, DIP, DSIPI, GILZ, TSC-22R, DSIP-immunoreactive leucine zipper protein|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.