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GILZ, PE, clone: CFMKG15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-4033-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15258759

  • £80.62 / 25µg


Estimated Shipment date
from Supplier 26-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB. GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predominantly cytoplasmic staining.Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.Applications Tested: This CFMKG15 antibody has been tested by intracellular flow cytometric analysis of dexamethasone-treated mouse lymph node cells and human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For intracellular staining with CFMKG15, it is recommended to use IC Fixation Buffer (cat. 00-8222) followed by permeabilization with a 1X dilution of the 10X Permeabilization Buffer (cat. 00-8333).Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GILZ
CFMKG15
PE
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Immunocytochemistry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Glucocorticoid-Induced Leucine Zipper, TSC22D3
IgG2a
RUO
Primary
Murine
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