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Gibco™ RPMI 1640 Medium (Dutch modification)

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 22409015

13.60 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.50000kg

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Product Code. 10575573

  • £20.41 / 500mL
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For Research Use Only. All usage must comply with product instructions.
 

Requires : Supplementation

Description

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: Phenol Red, HEPES, Low Sodium Bicarbonate
Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has the Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1 g/L).

Product Intended Use
For Research Use Only. Not for use in diagnostic procedures.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (1.0 g/L) and therefore requires a 5-7% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Specifications

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Leukemic Cells
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterile-filtered
No Glutamine, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
500 mL
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Liquid
Standard Serum Supplementation
HEPES, Phenol Red, Low Sodium Bicarbonate
Sustainable packaging
Gibco™
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.