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Gibco™ RPMI 1640 Medium (Dutch modification)
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Brand: Gibco™ 22409015
13.60 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : 50
Additional Details : Weight : 0.50000kg
Requires : Supplementation
Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With: Phenol Red, HEPES, Low Sodium Bicarbonate
Without: L-glutamine
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has the Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1 g/L).
Product Intended Use
For Research Use Only. Not for use in diagnostic procedures.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (1.0 g/L) and therefore requires a 5-7% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Specifications
Storage conditions: 2 – 8°C. Protect from light. Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
Leukemic Cells | |
1 X | |
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
Sterile-filtered | |
No Glutamine, No Sodium Pyruvate | |
cGMP-compliant under the ISO 13485 standard | |
500 mL |
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
Animal Origin-free | |
Liquid | |
Standard Serum Supplementation | |
HEPES, Phenol Red, Low Sodium Bicarbonate | |
Sustainable packaging | |
Gibco™ | |
Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.