Gibco™ RPMI 1640 Medium

Originally developed to culture human leukemic cells in suspension and as a monolayer

Brand:  Gibco™ 11875168

Product Code. 12559059

Description

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

• Modified as follows: with L-glutamine, phenol red; without HEPES
• Contains reducing agent glutathione and high concentrations of vitamins
• Contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• Vitamins inositol and choline are present in high concentrations
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Product Line: Gibco™, SynerGy™
• Serum Supplementation: Standard Serum Supplementation
• Volume (Metric): 5 L
• Glutamine: L-Glutamine
• Sodium Pyruvate Additive: No Sodium Pyruvate

• HEPES Buffer: No HEPES
• Quantity: 5L
• Shipping Condition: Room Temperature
• Form: Liquid
• Phenol Red Indicator: Phenol Red

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.