Gibco™ Medium 199, Earle's Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Brand:  Gibco™ 31150030

Product Code. 11554426

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light.
  Shipping conditions: Ambient.
  Shelf life: 12 months from date of manufacture.
• Cell Line: Rat epithelial
• Concentration: 1X
• Product Type: Medium 199
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
• Green Features: Sustainable packaging
• Osmolality: 290 - 310 mOsm/kg
• Quantity: 10 x 500 mL

• Culture Environment: CO₂
• Cell Type: Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.