Ham's F-12K (Kaighn's) Medium

A modification of Ham's F-12 Nutrient Mixture

Brand: Gibco

Manufacturer Part Number: 21127030


UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 6.12000kg

Product Code. 10667440

Quantity Price
1 £149.00 / Pack of 10
EU Stock 38
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Quantity 10 x 500mL
Storage Requirements Storage: 2-8°C. Protect from light. Shelf life : 12 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) Medium is modified as follows: with L-glutamine, phenol red; without HEPES.

  • Contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate
  • Additions allow medium to be supplemented with very low levels of serum or defined components, for some cell types
  • Contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS)
  • FBS concentration must be optimized for each cell line
  • Uses sodium bicarbonate buffer system (2.5g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging


Cell Culture, Mammalian Cell Culture

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