GFP, Biotin, clone: 5F12.4, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-6498-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This 5F12.4 monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.Applications Reported: This 5F12.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5F12.4 antibody has been tested by flow cytometric analysis of cells transiently transfected with a GFP-encoded expression construct. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|GFP2, Green Fluoresecent Protein, GFP, Turbo GFP, eGFP|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.