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GFP, clone: GF28R, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6674-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15557466

  • £43.22 / 25µg


EU Stock 1
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Description

Description

Description: This GF28R monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This 27-kDa protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.Applications Reported: This GF28R antibody has been reported for use in immunoprecipitation, and western blotting.Applications Tested: This GF28R antibody has been tested by immunoblotting of reduced cell lysate expressing GFP at less than or equal to 1 ug/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GFP
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
GFP2, Green Fluoresecent Protein, GFP, Turbo GFP, eGFP
IgG1
25μg
Store at 2-8°C.
Primary
Murine
ImmunoprecipitationþWestern Blot
GF28R
Unconjugated
Purified
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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