GE Healthcare StrepTactin™ Sepharose™ High Performance Medium

Mild purification conditions preserve antigenicity and function. GE Healthcare StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.

Overview
Brand: GE Healthcare

Manufacturer Part Number: 28935600

UNSPSC: 41116012

Code: GE

Additional Details:
Additional Details: Weight: 0.11400kg



Product Code. 10762827

Quantity Price
1 £ 2003.68 / 50mL
Estimated Shipment
16-05-2017
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Description and Specification

Specification

Flow Rate <300 cm/hr.
Storage Requirements 2° to 8°C
Product Type StrepTactin Sepharose HP media
Ligand Type StrepTactin (∼5mg StrepTactin/mL medium)
Particle Size 34μm
For Use With (Application) Purification of Strep (II) -tagged recombinant Proteins to high purities in concentrated forms and small volumes by using with perstaltic pump or chromato graphy system
pH Range 7
Quantity 50mL
Support Type 6% Highly Cross-linked Agarose

  • Medium with high binding capacity yields high resolution, sharp concentrated peaks, and reproducible purifications
  • Physiological conditions and mild elution preserves the activity of the target protein
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Easily scaled up, and easily regenerated with 0.5M NaOH
  • Convenient use with a peristaltic pump or chromatography system

  • Matrix: rigid, highly cross-linked 6% agarose
  • Ave. particle size: 34μm
  • Dynamic binding capacity (protein dependent): approx. 6mg Strep(II)-tagged protein/mL medium
  • Compatibility: stable in all commonly used buffers, 0.5M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents
  • pH stability: >7