Cytiva StrepTactin™ Sepharose™ High Performance Medium

Mild purification conditions preserve antigenicity and function. Cytiva StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.

Brand:  Cytiva 28-9355-99

Product Code. 10636895

Description

• Medium with high binding capacity yields high resolution, sharp concentrated peaks, and reproducible purifications
• Physiological conditions and mild elution preserves the activity of the target protein
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Easily scaled up, and easily regenerated with 0.5M NaOH
• Convenient use with a peristaltic pump or chromatography system

• Matrix: rigid, highly cross-linked 6% agarose
• Ave. particle size: 34μm
• Dynamic binding capacity (protein dependent): approx. 6mg Strep(II)-tagged protein/mL medium
• Compatibility: stable in all commonly used buffers, 0.5M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents
• pH stability: >7

Specifications

• Product Type: StrepTactin Sepharose HP media
• Flow Rate: <300 cm/hr.
• pH Range: 7
• Support Type: 6% Highly Cross-linked Agarose
• For Use With (Application): Purification of Strep (II) -tagged recombinant Proteins to high purities in concentrated forms and small volumes by using with perstaltic pump or chromato graphy system

• Quantity: 10mL
• Ligand Type: StrepTactin (∼5mg StrepTactin/mL medium)
• Particle Size: 34μm
• Content And Storage: 2° to 8°C
• dimMatrix: Strep-Tactin Sepharose

Safety and Handling

Product Identifier

• StrepTactin Sepharose High Performance, 10 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol