GE Healthcare Sepharose™ Fast Flow Cation Exchange Media

Used for preparative protein separations in both research and industrial applications. GE Healthcare CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17071901

500 ML CM SEPHAROSE(TM) FAST FLOW 500ML STORE ATambient Store at Room Temperature

UNSPSC: 41104018

Code: GE

Additional Details:
Additional Details: Weight: 1.58760kg



Product Code. 10714592

Quantity Price
1 £ 405.3 / 500mL
Estimated Shipment
05-01-2017
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Description and Specification

Specification

Stability Stable in commonly used aqueous Buffers: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Quantity 500mL
Storage Requirements 4 to 30°C
Anion Exchange Type Weak CatIon Exchanger
Anion Exchange Capacity 0.09 to 0.13mM Hl+/mL
Binding Capacity 50mg Ribonuclease A/mL medium
Product Type CM Sepharose fast flow media
For Use With (Application) For preparative Protein separations in research and industrial appliCatIons, for capture and intermediate purifiCatIon of wide range of approved biopharmaceuticals
Flow Rate 300 to 600cm/Hour
Particle Size 45 to 165μm
Max. Pressure 100kPa
Matrix 6% Cross-Linked Agarose
pH Range 4 to 13
Storage Buffer 20% Ethanol
Ligand Type Carboxymethyl
Packaging Quantity 500mL

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals