GE Healthcare Sepharose™ Fast Flow Cation Exchange Media

Used for preparative protein separations in both research and industrial applications. GE Healthcare CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

Brand: GE Healthcare

Manufacturer Part Number: 17072910


UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.11000kg

Product Code. 10576350

Quantity Price
1 £46.71 / 25mL
Estimated Shipment
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Description and Specification


Stability Stable in commonly used aqueous Buffers: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Quantity 25mL
Storage Requirements 4 to 30°C
Anion Exchange Type Strong CatIon Exchanger
Anion Exchange Capacity 0.18 to 0.25mM Hl+/mL
Binding Capacity 70mg Ribonuclease A/mL medium
Product Type SP Sepharose fast flow media
For Use With (Application) For preparative Protein separations in research and industrial appliCatIons, for capture and intermediate purifiCatIon of wide range of approved biopharmaceuticals
Flow Rate 400 to 700cm/Hour
Particle Size 45 to 165μm
Max. Pressure 100kPa
Matrix 6% Cross-Linked Agarose
pH Range 4 to 13
Storage Buffer 20% Ethanol, 0.2M Sodium Acetate
Ligand Type Sulphopropyl
Packaging Quantity 25mL

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals