GE Healthcare nProtein A Sepharose™ 4 Fast Flow Affinity Media

Used for purification of monoclonal and polyclonal antibodies.   GE Healthcare Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Brand: GE Healthcare

Manufacturer Part Number: 17-5280-02

UNSPSC: 41116126

Code: GX

Additional Details:
Additional Details: Weight: 1.33810kg

Product Code. 11369931

Quantity Price
1 £ 3282.5 / 200mL
Estimated Shipment
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Description and Specification


Binding Capacity >35mg Human IgG/mL
Flow Rate 150 to 250cm/Hour
For Use With (Application) For the purifiCatIon of monoclonal and polyclonal antibodies at both laboratory and process scale
Height (Metric) Bed 25cm
Ligand Type Native Protein A (S. aureus)
Matrix 4% Cross-Linked Agarose
Max. Pressure 100kPa
Quantity 200mL
Stability No significant change in chromatographic performance after 1 week storage using 8M Urea, 6M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
Storage Buffer 20% Ethanol
Storage Requirements 2 to 8°C
Particle Size 90μm
Product Type nProtein A Sepharose 4 fast flow media
pH Range 2 to 10

  • Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
  • Used in large-scale FDA-approved processes
  • Manufactured without using animal-derived components
  • Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
  • High chemical, thermal and mechanical stability
  • Withstands high concentrations of hydrogen bond disrupting agents