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GE Healthcare Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Brand:  GE Healthcare 17371201

UNSPSC : 41115700

Code : A0

Additional Details : Weight : 0.07200kg

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Product Code. 13415187

  • £293.68 / 25mL


Estimated Shipment
16-06-2017
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Specifications

Description

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Description & Specifications

Specifications

Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromatography (IMAC)
4° to 30°C
0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
25mL
6% Highly Cross-linked Agarose
150 to 600cm/hr.
Ni Sepharose excel chromato graphy media
90μm
2 to 12