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GE Healthcare illustra™ Single Cell GenomiPhi™ DNA Amplification Kit

Amplify whole genome DNA from as little as a single cell.  GE Healthcare Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

Brand:  GE Healthcare 29108039

UNSPSC : 12352200

Code : NEW

Additional Details : Weight : 0.00500kg Transport : UN number : 1814 Chem class : 1b Pack group : II

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Product Code. 15311652

  • £1053.08 / 100 reactions


Estimated Shipment
20-09-2017
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Description

Description

  • For whole genome amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
  • Reaction time: 2 to 4 hours
  • Product average size: >10kb
Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


Specifications

Specifications

illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
100 Reactions
Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Skin corrosion/irritation Category 1

Hazard Statement
  • H314-Causes severe skin burns and eye damage.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P331-Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P405-Store locked up.
  • P501-Dispose of contents/container to .