GE Healthcare illustra™ bacteria genomicPrep Mini Spin Kit

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria

Overview
Brand: GE Healthcare

Manufacturer Part Number: 28-9042-59

ILLUSTRA BACTERIA MINI (250)

UNSPSC: 41105500

Code: GE

Additional Details:
Additional Details: Weight: 1,395.00000kg



Product Code. 10546485

Quantity Price
1 £ 654.62 / 250 reactions
Estimated Shipment
15-12-2016
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Description and Specification

Specification

For Use With (Application) Rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteria
Includes 30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
Product Type Kit
Quantity 250 Preps

The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.

The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.

  • Total DNA extraction time under one hour with reduced lysis time
  • Can be used to purify 4 to 8μg of genomic DNA from 2 × 109 bacteria cells with input amounts ranging from 1 × 109 to 4 × 109 bacteria cells
  • Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
  • Validated in several downstream applications including RT PCR, long PCR, and restriction digest
  • Color-coded caps for ease of use