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GE Healthcare illustra™ bacteria genomicPrep Mini Spin Kit

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria

Brand:  GE Healthcare 28-9042-58

Code : GE

Additional Details : Weight : 0.47930kg

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Product Code. 10536485

  • £152.00 / Each


Estimated Shipment
06-07-2018
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Includes: illustra bacteria genomicPrep Mini spin columns prepacked with novel silica membrane; Lyophilized proteinase K powder; Lysis solutions, wash and elution buffers; 2mL Microcentrifuge collection tubes; a full booklet with a detachable, quick reference protocol card

Description

Description

The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.

The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.

  • Total DNA extraction time under one hour with reduced lysis time
  • Can be used to purify 4 to 8μg of genomic DNA from 2 × 109 bacteria cells with input amounts ranging from 1 × 109 to 4 × 109 bacteria cells
  • Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
  • Validated in several downstream applications including RT PCR, long PCR, and restriction digest
  • Color-coded caps for ease of use

Specifications

Specifications

Rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteria
2 x 30mg Proteinase K, 15mL Lysis Buffer type 2, 2 x 1.5mL Lysis Buffer type 3, 2 x 165mL Lysis Buffer type 4, 30mL Wash Buffer type 6, 60mL Elution Buffer type 5, 5 x 50 Illustra™ bacteria mini columns, 5 x 50 collection tubes
Kit
50 Preps
Videos
Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 3

Hazard Statement
  • H319-Causes serious eye irritation.
  • H315-Causes skin irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P405-Store locked up.
  • P501-Dispose of contents/container to .