GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Overview
Brand: GE Healthcare

Manufacturer Part Number: 28-9537-67

X5 HITRAP TALON CRUDE 5 ML

UNSPSC: 41115700

Code: A0

Additional Details:
Additional Details: Weight: 0.14600kg Transport: UN number : 1170 Chem class : 3 Pack group : III



Product Code. 12034118

Quantity Price
1 £ 425.75 / Pack of 5
In Stock 3
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Description and Specification

Specification

Quantity 5 x 5mL
Storage Requirements 4 to 8°C
Binding Capacity Up to 20mg Histidine-tagged Protein1
Product Type HiTrap Talon™ crude prepacked Column
Diameter (Metric) Inner 1.6cm
For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Flow Rate 1 to 4mL/min.
Particle Size 60 to 160μm
Material Polypropylene
Max. Pressure 3 bar
Matrix 6% Cross-Linked Agarose
pH Range 3 to 12
Storage Buffer 20% Ethanol
Ligand Type Talon™ Superflow Cobalt-IMAC
Packaging Quantity 5 x 5mL

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.