Cytiva HiTrap™ Protein A HP Prepacked Columns

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

Brand:  Cytiva 17-0403-01

Product Code. 11758438

Description

• Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
• For use with syringe, pump or chromatography system such as KTA design or FPLC System
• Binds 20mg human IgG/mL medium
• Stable over pH 3 to 9 for use and 2 to 10 for cleaning
• Designed for use with a syringe, a peristaltic pump, or a chromatography system
• Maximum pressure: 3 bar
• Column material: polypropylene
• 1 and 5mL columns

Media

• Ligand: Native protein A
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 9

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Binding Capacity: 20 mg/mL IgG Medium
• For Use With (Application): Chromatography System, Manual
• Content And Storage: 4°C to 8°C, 20% Ethanol

• Flow Rate: <20 mL/min.
• For Use With (Equipment): For syringe, peristaltic pump or a chromatography system
• Quantity: 1 x 5 mL

Safety and Handling

Product Identifier

• HiTrap Protein A 5 ml, 1 x 5 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 2

Hazard Statement

• H225-Highly flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol