Cytiva HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Brand:  Cytiva 17-0408-01

Product Code. 10605575

Description

• Uses different kinds of metal ions
• Binding capacity ∽12mg histidine-tagged protein/mL medium
• Metal ion capacity ∽23μmol Cu²⁺/mL medium
• Prepacked with Chelating Sepharose High Performance media
• Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

Specifications

• Buffer: 6M Guanidine Hydrochloride, 8M Urea
• Bed Volume: 1mL
• Diameter (Metric) Inner: 7mm
• Height (English): 0.98 in.
• Flow Rate: <4mL/min.
• Ligand Type: Iminodiacetic Acid
• pH Range: 3 to 13
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Affinity Column

• Product Type: HiTrap chelating HP column
• Diameter (English) Inner: 0.27 in.
• Quantity: 5 x 1mL
• Height (Metric): 25mm
• Material: Polypropylene
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Content And Storage: 4° to 30°C
• For Use With (Application): IMAC and subsequent purification of Polyhistidine tagged Proteins

Safety and Handling

Product Identifier

• HiTrap Chelating HP 1 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol