GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare Life Sciences™ HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17524801

HISTRAP HP 5ML

UNSPSC: 12352200

Code: GE

Additional Details:
Additional Details: Weight: 0.03680kg



Product Code. 11763209

Quantity Price
1 £ 125.86 / 1mL
In Stock 5
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Description and Specification

Specification

Storage Requirements 4° to 30°C
Diameter (Metric) Inner 16mm
Flow Rate 5mL/min.
Material Polypropylene
Product Type HisTrap HP Column
Packaging Quantity 1 x 5mL
Bed Volume 5mL
pH Range 3 to 12
Quantity 1 x 5mL
Support Type 6% Highly Cross-linked Agarose
Pressure 3 bar

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.