GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Brand: GE Healthcare

Manufacturer Part Number: 11-0004-58

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: CAS Number: 64-17-5 Weight: 0.10200kg



Product Code. 10685355

Quantity Price
1 £ 124.31 / Pack of 5
In Stock 2
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Description and Specification

Specification

Storage Requirements 4° to 30°C
Diameter (Metric) Inner 7mm
Flow Rate <4mL/min.
Material Polypropylene
Product Type HisTrap FF Crude Column
Packaging Quantity 5 x 1mL
Particle Size 45 to 165μm
Support Type 6% Cross-linked Agarose
Bed Volume 1mL
Includes Sodium Phosphate-buffered Saline, pH 7.4 and 0.5X concentration of proprietary Y-PER reagent additives
pH Range 3 to 12
Quantity 60mL
Pressure 3 bar

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.