GE Healthcare HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.   GE Healthcare Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Brand: GE Healthcare

Manufacturer Part Number: 17-3712-05

UNSPSC: 12161500

Code: GE

Additional Details:
Additional Details: CAS Number: 64-17-5 Weight: 0.10200kg

Product Code. 12632966

Quantity Price
1 £ 153.05 / 5mL
In Stock 4
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Description and Specification


Bed Volume 1mL
Diameter (Metric) Inner 7mm
Flow Rate 150 to 600cm/hr.
Material Polypropylene
Packaging Quantity 5 x 1mL
pH Range 2 to 12
Product Type HisTrap Excel Column
Pressure 5 bar
Quantity 5 x 1mL
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.