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GE Healthcare His Mag Sepharose™ excel Chromatography Media

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Brand:  GE Healthcare 17371221

Code : GX

Additional Details : CAS Number : 79-06-1 Weight : 0.08800kg

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Product Code. 12601216

  • £211.00 / Pack of 5


Estimated Shipment
11-06-2018
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Description

Description

Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.

  • His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
  • The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
  • Simplify handling using visible and dense Sepharose-based magnetic beads.
  • Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
  • The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
  • The base matrix is based on Sepharose medium with magnetite incorporated.
  • His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.

Specifications

Specifications

Designed to simplify small-scale purification and screening of His-tagged Proteins_x000D_ secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
63μm
5 x 1mL
Highly cross-linked spherical Agarose including Magnetite
2 to 12
His Mag Sepharose Excel
4° to 30°C
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .