Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Product Code	Brand	Quantity
11575125	Cytiva 28-4017-48	5 x 5 mL
11743319	Cytiva 28-4017-47	1 x 5 mL

Description

• Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
• May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
• Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.
• Average particle size: 90μm

Media

• Matrix: Highly cross-linked 4% agarose
• Binding capacity: >25mg horse liver GST/mL
• pH stability working range: 4 to 13

Specifications

• Bed Volume: 5 mL
• Diameter (Metric) Inner: 16 mm
• Flow Rate: <20 mL/min.
• pH Range: 4 to 13
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 45 to 165 μm
• Type: Pre-packed Column

• Diameter (English) Inner: 0.62 in.
• Description: GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
• Material: Polypropylene
• Support Type: 4% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• For Use With (Application): GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
• Ligand Type: Glutathione