GE Healthcare GST Gene Fusion System pGEX Vectors

Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST

Overview
Brand: GE Healthcare

Manufacturer Part Number: 28954553

25 UL PGEX-5X-1 25µL STORE AT -20°C

UNSPSC: 41106600

Code: GE

Additional Details:
Additional Details: Weight: 0.01200kg



Product Code. 10534625

Quantity Price
1 £ 384.95 / 25µL
Estimated Shipment
27-01-2017
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Description and Specification

Specification

For Use With (Application) Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST
Includes 5X-1
Product Type pGEX vecto r
Quantity 25μg

  • Offers a tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins
  • Offers an internal laq1q gene for use in any E. coli host
  • With PreScission™ Protease, Thrombin, or Factor Xa recognition sites for cleaving the target protein from the fusion product
  • The vectors provide all three translational reading frames beginning with the EcoRI restriction site
  • Can use a wide variety of E. coli host strains cloning and expression

  • pGEX-1lT EcoRI/BAP is an EcoR1 pre cut vector, phosphatase treated to prevent self ligation, ready for gene insertion
  • pGEX-2TK will introduce a specific kinase site allowing the fusion protein to be labelled with [γ-32P]ATP using protein kinase