RNAi experiments, positive correlation of cytoplasmic Fluorescence with transfection efficiency, silences the Cyclophilin B gene and is labeled with DY-547
Visual confirmation of transfection efficiency using conventional fluorescent labeled siRNA often does not correlate with siRNA uptake and gene silencing. This is due to several factors including the cleavage of the fluorescent label from the siRNA and rapid photo-bleaching of the fluorophore. In addition, when used in co-transfection experiments, evidence suggests that fluorescently labeled siRNA may compete with functional siRNA for the RISC if the targeting siRNA is not of optimal design, thus reducing silencing efficiency.
siGLO Lamin A/C siRNA is a stable, fluorescent form of Lamin A/C that enables correlation of fluorescence uptake with silencing activity. Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamin A/C is abundantly expressed in most human, mouse and rat cells and knockdown of the corresponding mRNA does not affect cell viability.
These control siRNAs are chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores. Each siRNA is designed to target a site within the respective gene that is identical for both human, mouse and rat.