Determination of baseline cellular responses in RNAi experiments
BLAST analysis confirms at least 4 mismatches with all known human, mouse and rat genes
Characterized by microarray analysis
Minimal off-target effects
The siGENOME Non-Targeting Pools are each comprised of four siGENOME Non-Targeting siRNAs, and are recommended for experiments using pooled siRNA such as Dharmacon SMARTpool reagents. Each individual siRNA within this pool was characterized by genome-wide microarray analysis and found to have minimal off-target signatures. siGENOME Non-Targeting Pool #1 is comprised of Non-Targeting siRNAs #1-4
siGENOME Non-Targeting Pool #2 is comprised of Non-Targeting siRNAs #2-5
Please Note: Customer reports have shown that siGENOME Non-targeting siRNA #1 reduces EGFR mRNA (NM_005228) in an assay- and cell-specific manner by ∽50%. This finding was not observed by initial microarray validation, but has been subsequently verified by internal scientists.
siGENOME Non-Targeting siRNA #2 and #4 target firefly luciferase (U47296).