Gata-3 Rat anti-Human, Mouse, PE-eFluor 610, Clone: TWAJ, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 61-9966-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15540587

Quantity Price
1 £ 84.99 / 25 tests
Estimated Shipment date
from Supplier 02-05-2017
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Description and Specification

Specification

Regulatory Status RUO
Species Reactivity Human, Mouse
Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Applications Flow Cytometry (Intracellular Staining)
Clone TWAJ
Isotype IgG2b
Primary or Secondary Primary
Conjugate PE-eFluor 610
Concentration 5μL (0.03μg)/test
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Quantity 25 tests
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Antigen Gata-3

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.