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GARP Rat anti-Mouse, PE-Cyanine7, Clone: YGIC86, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-9891-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15588686

  • £91.75 / 25µg

Estimated Shipment
06-01-2021

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Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.
Specifications

Specifications

GARP
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Lrrc32
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
434215
Flow Cytometry
YGIC86
PE-Cyanine7
Lrrc32
D11S833E, GARP, garpin, glycoprotein A repetitions predomit, leucine-rich repeat-containing protein 32
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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For Research Use Only.