GARP, PE-Cyanine7, clone: YGIC86, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 25-9891-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin). GARP is an approximately 80kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta.When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody. Fixation/permeabilization is not necessary when staining Treg cells.YGIC86 does not cross-react to rat GARP.Applications Reported: This YGIC86 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YGIC86 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|