GARP Rat anti-Mouse, eFluor 450, Clone: YGIC86, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-9891-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15391830

Quantity Price
1 £ 84.99 / 25µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Gene Alias Lrrc32, Garpin
Format Conjugated
Monoclonal or Polyclonal Monoclonal
Isotype IgG2a
Antigen GARP
Clone YGIC86
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Quantity 25μg
Host Species Rat
Applications Flow Cytometry
Species Reactivity Mouse
Conjugate eFluor 450
Regulatory Status RUO
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL

The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin). GARP is an approximately 80kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta.

When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody. Fixation/permeabilization is not necessary when staining Treg cells.

YGIC86 does not cross-react to rat GARP.